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LINKS
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SISTEMI DI SMASCHERAMENTO
ANTIGENICO
Antigen Retrieval
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nessun
trattamento |
| C1-3 pH6
C1-3 pH7
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Trattamento ad alte temperature in forno MW
in tampone citrato (1-3 si riferisce al tempo) |
| PP pentola a pressione |
Bollitura in PP per tempi e pH differenti |
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| Trattamento con enzimi proteolitici |
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Antigen retrieval immunohistochemistry and molecular
morphology in the year 2001.
Shi SR, Cote RJ, Taylor CR.
Appl Immunohistochem Molecul Morphol 2001 Jun;9(2):107-16
Department of Pathology, University of Southern California Keck School
of Medicine, Los Angeles 90033, USA.
The impact of the antigen retrieval (AR) technique upon diagnostic
immunohistochemistry (IHC) and upon research has been demonstrated by
numerous of articles and more than a dozen major reviews. The specific aim
of this survey of the field is to examine potential new approaches to
retrieval of nucleic acid and protein from archival paraffin-embedded tissue
for molecular biology-based diagnostic procedures that form the basis of the
emerging field of molecular morphology. Any new approach must incorporate
the principles of standardization and improved reproducibility. The ultimate
goal will be to understand the mechanisms of fixation (by formalin) and
"unfixation" (by AR). In the interim, the diligent application of
practical procedures that have been shown to be tried and true is the least
that we must demand from ourselves and our laboratories.
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