L'analisi dell'espressione di CD16 nei processi linfoproliferativi è di solito effettuata mediante analisi immunofenotipica in citometria a flusso. La disponibilità di un anticorpo utilizzabile su materiale istopatologico (incluso in paraffina)  rende possibile la ricerca di CD16 su materiale di routine per caratterizzare le neoplasie T/NK (linfonodo, biopsia osteomidollare, polmone, etc.).

figura 1. intensa espressione di CD16 nelle cellule macrofagiche presenti 

nella polpa rossa splenica

figura 2. intensa espressione di CD16 nelle cellule macrofagiche presenti 

in un linfoma T (CD16-).

figura 3. linfoma T/NK cutaneo CD16+

figura 4.  linfoma T/NK del polmone (nasal-type) CD16+

 

 

Int J Cancer. 1999 Jun 11;81(6):865-70. 

Immunophenotypic and genotypic characterization of nasal lymphoma with polymorphic reticulosis morphology. 

Ohsawa M, Nakatsuka S, Kanno H, Miwa H, Kojya S, Harabuchi Y, Yang WI, Aozasa K. Department of Pathology, Osaka University Medical School, Suita, Japan. 

Nasal lymphoma with polymorphic reticulosis (PR) morphology is now categorized as T/natural killer (T/NK) cell lymphoma. In this study, immunophenotypes and genotypes of proliferating cells in 21 cases with PR were examined. The patients included 13 men and 8 women ranging in age from 20 to 74 (median 37) years. All patients presented with lesions in the upper respiratory tract, mostly in the nasal cavity. Histological specimens obtained from the primary lesions (19 cases) and metastatic cervical lymph nodes (2 cases) were used for analyses. Histologically, polymorphous proliferation was found in 20 cases, and these were thus diagnosed as PR. A monomorphous pattern was found in the remaining last case. Immunohistochemical analysis revealed that the proliferating cells were CD56 (123C3)+ and/or CD16 (2H7)+, TIA-1+ and frequently stained CD3 epsilon+. Tumor cells were frequently stained positively with monoclonal antibodies (mAbs) for T lymphocytes, but were negative for T-cell receptor (TCR) beta and delta chain expression. In situ hybridization analysis using an Epstein-Barr virus-encoded early RNA 1 (EBER-1) probe revealed positive signals in 13 of the 15 cases examined. Southern blotting analysis for clonality of the Epstein-Barr virus (EBV) genome in 12 positive cases confirmed the presence of monoclonal proliferation in 7 cases. The pattern of TCR gamma chain gene rearrangement was examined by PCR analysis of DNA from tumor tissues by the denaturing gradient gel electrophoresis method. The results demonstrated no clonal rearrangement in any of the 21 cases examined, including 7 cases with proven clonal proliferation of EBV-infected cells, indicating the absence of T-cell clones. Our findings strongly suggested that nasal T-cell lymphoma is in fact a NK cell lymphoma. PMID: 10362131 [PubMed - indexed for MEDLINE]

 

Pathol Res Pract. 2002;198(5):369-73.

Primary bony peripheral T-cell lymphoma mimicking nasal type NK/T-cell lymphoma: a case report.

Chuang SS, Lin CN, Li CY.

Department of Pathology, Chi-Mei Medical Center, Yung-kang City, Tainan, Taiwan. ssc6061@ms16.hinet.net

Primary bony lymphomas are rare, and nearly all are high-grade B-cell lymphomas. Natural killer (NK)/T-cell lymphomas are highly aggressive lymphomas of NK- or T-cell lineage with predominant extranodal presentation and are divided into nasal and nasal-type (extra-nasal). We report a primary bony peripheral T-cell lymphoma mimicking NK/T-cell lymphoma, nasal type. A 22-year-old Taiwanese male presented with a frontal skull bone mass noted for 3 weeks, and received craniectomy with tumor removal. His tumor showed extensive coagulative necrosis with angioinvasion by large lymphoma cells expressing CD2, CD8, CD16, CD43, CD45, CD45RO, CD56, T-cell intracellular antigen-1, and granzyme B, but not CD3, CD4, CD20, CD57, CD68, and betaF1. In situ hybridization for Epstein-Barr virus-encoded mRNA was negative. Polymerase chain reaction study of formalin-fixed tissue showed clonal rearrangement of the T-cell receptor-gamma chain gene. The diagnosis was peripheral T-cell lymphoma, unspecified subtype. The initial stage was I(EA). His lymphoma was refractory to chemotherapy, and bony metastases developed in the right iliac bone 2 months later. He died of disease after 6 months without autopsy. We emphasize the importance of detailed immunohistochemical and gene rearrangement studies for the classification of malignant lymphomas via a very rare primary bony lymphoma of peripheral T-cell subtype.

PMID: 12092774 [PubMed - indexed for MEDLINE]